BT2307 Molecular Biology Anna University Lab manual - Computer Programming


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Sunday, July 3, 2011

BT2307 Molecular Biology Anna University Lab manual


 At the end of this course, the students would have learnt basic techniques used
in Molecular Biology and its application. This will be strength for students to
undertake research projects in the area of moderabiology.
1. Isolation of bacterial DNA
2. Isolation of plant cell and animal cell genomic DNA
3. Agarose gel electrophoresis
4. Restriction enzyme digestion
5. Competent cells preparation
6. Transformation and screening for recombinants
7. Agarose gel electrophoresis
8. Restriction enzyme digestion
9. Competent cells preparation
10. Blue and white selection for recombinants
11. Plating of Ophage
12. O phage lysis of liquid cultures

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