ABSORPTION SPECTRUM OF FERROUS IONS BY USING 1, 10- PHENONTHROLINE - Computer Programming

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Friday, April 1, 2011

ABSORPTION SPECTRUM OF FERROUS IONS BY USING 1, 10- PHENONTHROLINE


ABSORPTION SPECTRUM OF FERROUS IONS BY USING 1, 10- PHENONTHROLINE.
Aim:
To determine the λmax of Fe3+ - Phenonthroline complex by using absorption spectrum.
Principle:
λmax is the wavelength at which maximum absorption of the radiation takes place. A substance may have more than one λmax . In general, increase in concentration increases absorbance of a solution but, λmax is independent of concentration i.e. λmax will not vary with change in concentration. But λmax value of a substance will vary with change in solvents. λmax value is very characteristic of a substance and hence it is used for identification of substance.
 The reaction of ferrous Ammonium Sulphate and 1, 10 – Phenonthroline to form a complex which absorbs in UV and Visible region.
Chemicals required:
1.      Ferrous Ammonium Sulphate – 0.02M (dissolved water (ph= 4.5) as a solvent and add 1ml Conc. Sulphuric acid)
2.      1, 10 – Phenonthroline – 0.02M (dissolved in 0.2 w/v% in alcohol).
3.      Distilled water
Apparatus Required:
1.      Spectrophotometer/ Colorimeter
2.      Glass Cuvettes 
3.      Standard flasks, etc
Procedure:
1.      A series of three test tubes were labeled as A (Ferrous Ammonium Sulphate), B (1, 10 – Phenonthroline), and C (Ferrous Ammonium Sulphate +1, 10 – Phenonthroline).
2.      The 5ml of 0.02M Ferrous Ammonium Sulphate was pipetted out into the tube A and 5ml of 0.02M 1, 10 – Phenonthroline into tube B and pipetted out into the tube C and the contents of these tubes were mixed well with the help of cyclomixer.
3.      The maximum absorbance of these solutions were recorded at different wavelengths (430, 470, 520, 550, 580, 610, 700nm) using water as a blank.
4.      Three different spectral graphs were plotted (wavelength against absorption) from this λmax for Ferrous Ammonium Sulphate, 1,10- Phenonthroline and Ferrous Ammonium Sulphate- 1,10- Phenonthroline complex was calculated.


Result:
1.      Maximum absorption (λmax) for Ferrous Ammonium Sulphate is………..
2.      Maximum absorption (λmax) for 1, 10- Phenonthroline is………..
3.      Maximum absorption (λmax) for Ferrous Ammonium Sulphate- 1, 10- Phenonthroline complex is……………
Absorption Spectrum of Ferrous Ammonium Sulphate- 1, 10- Phenonthroline complex
S.
No
Wavelength
Absorption of Ferrous Ammonium Sulphate
Absorption of 1, 10- Phenonthroline
Absorption of Ferrous
Ammonium Sulphate-
1, 10- Phenonthroline complex
1
430



2
470



3
490



4
520



5
550



6
580



7
610



8
700





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